Granulocyte colony-stimulating factor (GCSF) is a glycoprotein that contains growth factors and aids in endometrial tissue development. The patient must have a receptive endometrium, as well as several other characteristics, for IVF treatment to be successful. Despite the multiple treatments available today for endometrial growth, no optimum medicine or strategy for enhancing endometrial density is available.
G-CSF (granulocyte colony-stimulating factor) is a glycoprotein that belongs to the colony-stimulating factor family. It aids in the formation of granulocyte colonies. Many cells, including endothelial monocytes and endometrial cells, produce G-CSF, which is an amino acid polypeptide. Endometrial decidualization, trophoblast development, and placental metabolism are all aided by G-CSF. G-CSF is involved in stem cell mobilization, migration, and differentiation. It also promotes endometrial regeneration by increasing angiogenesis and decreasing endometrial cell apoptosis.
Before the embryo transfer, GCSF is commonly injected intrauterine or administered subcutaneously, and it has been shown to help with implantation failures caused by thin endometrium. Experts usually administer one or two intrauterine G CSF applications. If the endometrium remains resistant, intrauterine platelet-rich plasma, or PRP, may be used. Thirty milliliters of blood are to be extracted from individuals in this procedure. FDA-approved equipment is used to process this blood. About 3-4 ml of PRP is to be collected from this. PRP is injected into the uterine cavity in a dose of 0.5 mL.
Injection GCSF is more effective than PRP in boosting endometrial density in patients with minimal endometrium. In a study, patients who received injection GCSF had a slightly higher clinical pregnancy rate (44%) than those who received intrauterine PRP, which had a rate of 28 percent. All of the other variables were similar.
The GCSF Instillation method provides the following benefits:
Increases Implantation Rates: GCSF instillation significantly improves the chances of embryo implantation into the endometrium by promoting the development of a receptive endometrium.
Improves Endometrial Thickness and Quality: GCSF Instillation improves the thickness and quality of the endometrium, which is essential for implantation, resulting in successful IVF treatment.
Is Safe and Non-Invasive: GCSF Instillation is a safe, minimally invasive procedure that does not interfere with the patient’s hormones.
Offers Hope to Patients with Repeated Implantation Failure: Patients with repeated implantation failures often feel hopeless but can now use GCSF instillation to improve their chances of successful implantation.
Step 1: Evaluation and Preparation
The first step involves thoroughly evaluating the patient’s medical history, gynaecological assessment, and uterine evaluation using ultrasound. After the evaluation, the patient’s ovaries are stimulated with medication to encourage the development of follicles and prepare the endometrium for implantation.
Step 2: GCSF Instillation
The next step involves administering GCSF into the uterus by a trained specialist using a catheter. The solution is delivered directly into the uterus to improve the endometrial lining’s thickness and quality.
Step 3: Follow-up
After the procedure, the patient is monitored closely for any complications. The doctor schedules regular follow-up appointments to determine the effectiveness of the therapy and adjust accordingly.
PRP is a concentrated blood product that contains a high percentage of platelets (biomolecules with natural healing and regenerative properties) In general, whole blood is composed of 4 main components: Plasma: the liquid component of blood that consists mostly of water, dissolved salts and proteins. It constitutes more than half of blood volume and provides the medium for all other components in the blood to be transported throughout the body. Platelets: Also known as thrombocytes, platelets are cell-like particles that help in the clotting process and release other substances and growth factors. Red Blood Cells: Also called erythrocytes, red blood cells carry oxygen is involved in the exchange of Oxygen and Carbon Dioxide, brining oxygen to tissue, and removing carbon dioxide from the body. White Blood Cells: Also known as leukocytes) are white blood cells are primarily responsible for the body’s immune system.
PRP is most often used from the person’s blood undergoing the PRP therapy but can be made from another individual’s blood. Step 1: The PRP process starts with a healthcare professional drawing a sample of blood similar to how they would normally collect tubes of blood. Step 2: The blood is centrifuged (put in a machine that spins very quickly causing the different components of blood to separate) for about 15 minutes. Step 3: A lab technician collects the plasma (now rich in platelets and devoid of cellular components) in preparation for its injection.
In comparison to intrauterine PRP infusion, injection GCSF is more successful in the treatment of thin endometrial patients. Despite the fact that the clinical and chemical pregnancy rates were comparable, the injection GCSF group had a larger percentage of clinically pregnant women. For thin endometrium, intrauterine PRP can be a useful alternative.